Computational and Quantitative Biology PhD

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    Omics strategies for studying gene expression ad DNA damage 2023

    Omics strategies for studying gene expression ad DNA damage

     

    Period

    3rd-11th May 2023

     

    Lecture

    Susanna Ambrosio

     

    Teams Code

    tuapci5

     

    Title

    Omics strategies for studying gene expression ad DNA damage (1CFU)

    PhD in Computational Quantitative Biology

     

    This course is designed for students of computational biology PhD who want to gain an integrated understanding of the latest genomic techniques for studying gene expression and DNA damage.

    By the end of the course, the students will be equipped with a basic theoretical knowledge of the wet lab techniques underlying the omics strategies, allowing them to have a more complete understanding of the entire process of genomic research, from sample preparation to data analysis and interpretation.

     

    Program

    Lesson 1: 3rd May 10:00-12:00

    • Introduction to omics strategies; chromatin structure and epigenetic modifications. Epigenomic analysis for the study of DNA and histone modifications (MeDIP-seq, DIP-seq ChIP-seq).

    Lesson 2: 4th May 10:00-12:00

    • Gene expression analysis: RNA-seq; GRO-seq; ATAC-seq.

    Lesson 3: 10th May 10:00-12:00

    • Introduction to types of damage and repair mechanisms.

    Lesson 4: 11th May 10:00-12:00

    • DNA damage genomics: AP-seq, SAR-seq, BLESS, END-seq. Techniques for inducing and visualizing site-specific DNA damage (endonucleases; CRISPR-mediated).

     

     

     

     

     

     

     

     

     

     

     

     

     

     

     

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