Omics strategies for studying gene expression ad DNA damage

 

Period

3^rd-11^th May 2023

 

Lecture

Susanna Ambrosio

 

Teams Code

tuapci5

 

Title

Omics strategies for studying gene expression ad DNA damage (1CFU)

PhD in Computational Quantitative Biology

 

This course is designed for students of computational biology PhD who want to gain an integrated understanding of the latest genomic techniques for studying gene expression and DNA damage.

By the end of the course, the students will be equipped with a basic theoretical knowledge of the wet lab techniques underlying the omics strategies, allowing them to have a more complete understanding of the entire process of genomic research, from sample preparation to data analysis and interpretation.

 

Program

Lesson 1: 3^rd May 10:00-12:00

• Introduction to omics strategies; chromatin structure and epigenetic modifications. Epigenomic analysis for the study of DNA and histone modifications (MeDIP-seq, DIP-seq ChIP-seq).

Lesson 2: 4^th May 10:00-12:00

• Gene expression analysis: RNA-seq; GRO-seq; ATAC-seq.

Lesson 3: 10^th May 10:00-12:00

• Introduction to types of damage and repair mechanisms.

Lesson 4: 11^th May 10:00-12:00

• DNA damage genomics: AP-seq, SAR-seq, BLESS, END-seq. Techniques for inducing and visualizing site-specific DNA damage (endonucleases; CRISPR-mediated).